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We investigated the mechanisms of tumor necrosis factor-alpha (TNF-alpha)-induced interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) expression in human monocytic cell lines (THP-1 and U937). Pretreatment of cells with various inhibitors of protein kinase C (PKC) (chelerythrine and H-7), tyrosine kinases (genistein and herbimycin A), and nitric oxide synthase (N-methyl-L-arginine and NG-monomethyl-L-arginine) abrogated TNF-alpha-induced IL-8 and ICAM-1 expression. Although pretreatment with tyrosine kinase inhibitors did not affect TNF-alpha-induced IL-8, ICAM-1, and O2- production, pretreatment with PKC inhibitors abolished both IL-8 and ICAM-1 expression as well as O2- production. Thus, we suggest that tyrosine phosphorylation, phosphoinositide 3-kinase, and PKC activation are indispensable for TNF-alpha-induced IL-8 and ICAM-1 expression.[Is an endonasal endoscopic approach better than a transsphenoidal approach for pituitary adenomas?].
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